Akademik Veri Yönetim Sistemi
1st International Gobeklitepe Agriculture Congress, Şanlıurfa, Türkiye, 25 - 27 Kasım 2019, cilt.1, sa.1, ss.113, (Özet Bildiri)
Norduz and Karakaş sheep have been adapted to Lake Van region
for many years as being domestic genetic resources and
meat-milk-offspring-fleece traits are a reliable economic resource especially
preferred by small family businesses around the area.
The PCR-RFLP technique is used as polymorphic genetic markers in the
determination of quantitative traits or diseases determined by functional genes
in farm animals as much as plants and humans. As polymorphisms is detected, it
is possible to select individuals carrying the desired genotypes by making use
of the statistical relationships between genetic markers and traits. In this
study, milk protein genes, casein (CSN3), lactoalbumin (LALBA1,2) variants and
lactoglobulin (LGB) of Norduz (n = 27) and Karakaş (n = 36) sheep breeds found
in Van-Yüzüncü Yıl University, Faculty of Agriculture, Livestock Research and
Application Farm were examined based on PCR-RFLP technique. As a result, CSN3
(769 bp) and LBG (849bp) gene regions were not be amplified by PCR, whereas PCR
products were obtained from LALBA1 (693 bp) and LALBA2 (843bp) gene regions.
However,
the expected sizes of alleles of LALBA1 digested with EcoR1 and LALBA2
digested with HindIII could not be observed, therefore there were no
polymorphisms detected in individuals under investigation. This result has
moved the aim of the research to the next stage and directed to Single
Nucleotide Polymorphisms (SNP), which is a more reliable method for the
detection of polymorphisms in other related loci such as growth differentiation
factors (GDF) gene of milk protein genes.
Keywords:
Norduz and Karakaş sheep, milk protein genes, PCR-RFLP, genetic polymorphisms