Determination of Milk Protein Polymorphism in Norduz and Karakaş Sheep Breeds using PCR-RFLP Technique


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Koyun H. , Kiraz S., Karaca S. , Yılmaz A., Karakuş K., Yeşilova A. , ...Daha Fazla

1st International Gobeklitepe Agriculture Congress, Şanlıurfa, Türkiye, 25 - 27 Kasım 2019, cilt.1, no.1, ss.113

  • Cilt numarası: 1
  • Basıldığı Şehir: Şanlıurfa
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.113

Özet

Norduz and Karakaş sheep have been adapted to Lake Van region for many years as being domestic genetic resources and meat-milk-offspring-fleece traits are a reliable economic resource especially preferred by small family businesses around the area. The PCR-RFLP technique is used as polymorphic genetic markers in the determination of quantitative traits or diseases determined by functional genes in farm animals as much as plants and humans. As polymorphisms is detected, it is possible to select individuals carrying the desired genotypes by making use of the statistical relationships between genetic markers and traits. In this study, milk protein genes, casein (CSN3), lactoalbumin (LALBA1,2) variants and lactoglobulin (LGB) of Norduz (n = 27) and Karakaş (n = 36) sheep breeds found in Van-Yüzüncü Yıl University, Faculty of Agriculture, Livestock Research and Application Farm were examined based on PCR-RFLP technique. As a result, CSN3 (769 bp) and LBG (849bp) gene regions were not be amplified by PCR, whereas PCR products were obtained from LALBA1 (693 bp) and LALBA2 (843bp) gene regions. However, the expected sizes of alleles of LALBA1 digested with EcoR1 and LALBA2 digested with HindIII could not be observed, therefore there were no polymorphisms detected in individuals under investigation. This result has moved the aim of the research to the next stage and directed to Single Nucleotide Polymorphisms (SNP), which is a more reliable method for the detection of polymorphisms in other related loci such as growth differentiation factors (GDF) gene of milk protein genes.

Keywords: Norduz and Karakaş sheep, milk protein genes, PCR-RFLP, genetic polymorphisms