Bioguided Isolation of Secondary Metabolites from Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa


Ertaş A., Çakırca H., Yener I., Akdeniz M., Fırat M. , Topçu G., ...More

RECORDS OF NATURAL PRODUCTS, vol.15, no.6, pp.585-592, 2021 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 15 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.25135/rnp.248.21.01.1933
  • Title of Journal : RECORDS OF NATURAL PRODUCTS
  • Page Numbers: pp.585-592

Abstract

In the current study, the ethanol extracts prepared from the aerial parts and roots of an endemic

species, Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa were fractionated on silica gel columns and tested

for determination of their antioxidant activity using DPPH free radical and ABTS cation radical scavenging, and

cupric reducing antioxidant capacity (CUPRAC) test assays. Twenty known secondary metabolites were isolated

from the active antioxidant fractions; rosmarinic acid (1), chlorogenic acid (2), caffeic acid (3), 4-

hydroxybenzoic acid (4), benzoic acid (5), luteolin 7-O-glucoside (6), bis-(2-ethylhexyl)benzene-1,2-

dicarboxylate (7), salvianolic acid A (8), salvianolic acid B (9), 7-acetylroyleanone (10), 6,7-dehydroroyleanone

(11), ferruginol (12), inuroyleanol (13), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (14), ursolic acid

(15), oleanolic acid (16), taraxasterol (17), lupenone (18), β-sitosterol (19), and stigmasterol (20). Rosmarinic

acid, which was obtained from the aerial parts, was found to be the best antioxidant compound among the

isolated secondary metabolites in DPPH free radical and ABTS cation radical scavenging, and CUPRAC assays

(IC50: 1.20±0.04 μg/mL, IC50: 1.74±0.06 μg/mL, A0.5: 1.22±0.02 μg/mL, respectively). Chlorogenic and caffeic

acids, luteolin 7-O-glucoside, salvianolic acids A and B, and inuroyleanol exhibited also high antioxidant

activity in the mentioned assays.