Assesment of hematotoxic, oxidative and genotoxic damage potentials of fipronil in rainbow trout Oncorhynchus mykiss, Walbaum

UÇAR A., PARLAK V., Cilingir Yeltekin A. Ç., ÖZGERİŞ F. B., ÇAĞLAR Ö., TÜRKEZ H., ...Daha Fazla

TOXICOLOGY MECHANISMS AND METHODS, cilt.31, sa.1, ss.73-80, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1080/15376516.2020.1831122
  • Dergi Adı: TOXICOLOGY MECHANISMS AND METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.73-80
  • Anahtar Kelimeler: Fipronil, O, mykiss, hematology, enzyme parameters, 8-OHdG, caspase-3, MICRONUCLEUS TEST, COPPER-SULFATE, DNA-DAMAGE, ANTIOXIDANT, LIVER, EXPOSURE, DELTAMETHRIN, ERYTHROCYTES, COMBINATION, PARAMETERS
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

In this study, changes in the blood tissue of rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) caused by Fipronil (FP) insecticide were investigated using different biomarkers (Hematology parameters, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA), paraoxonase (PON), arylesterase (ARE), myeleperoxidase (MPO), micronucleus (MN), 8-hydroxy-2-deoxyguanosine (8-OHdG)) level and caspase-3 activity. Statistically significant alterations in hematology parameters occurred with FP effect. In blood tissue, dose-dependent inhibition was determined in SOD-CAT-GPX-PON and ARE enzyme activities, but MDA and MPO were induced statistically significant. The results of MN assay were compared with the control group and it was obtained that genotoxicity of different dose groups was similar. The level of 8-OHdG and the activity and caspase-3 examined in blood tissue was increased depending on the dose. It was determined with different biomarkers that this insecticide caused physiological stress changes in the tissues examined.