Background The aim of this study was to compare enamel colour changes of incisors and canines produced by various orthodontic adhesives following treatment with fixed appliances. Methods The study included 300 maxillary and mandibular central and lateral incisors, and canines of 25 subjects who finished fixed appliance treatment. The following adhesives were used according to the manufacturer's instructions: Transbond XT (3M Unitek, Monrovia, CA, USA), Kurasper F (Kuraray Europe GmbH, Frankfurt, Germany), Grengloo (Ormco Corporation, Glendora, CA, USA) and Light Bond (Reliance Orthodontic Products, Itasca, IL, USA). Different adhesives were placed in each quadrant of the subjects. Pretreatment and posttreatment enamel colours were evaluated at the middle third of the buccal surfaces of teeth using the SpectroShade Micro Dental Colour Complete Tooth Analysis System(TM) (MHT International, Verona, Italy). Tooth colour was determined using the Commission Internationale de l'Eclairage L* a* b* system, and increment E values were calculated. Results Pretreatment and posttreatment intragroup comparison results showed significant decreases in L* and b*, as well as insignificant changes in a* for all teeth. Significantly less enamel colour changes occurred in the Kurasper F group compared with the Grengloo and Light Bond groups. Evaluating incisors and canines separately, overall intergroup comparisons were not significant. Enamel colour changes were significantly less only for the canines compared to the incisors in the Transbond XT and Kurasper F groups. The increment E values were 1.83-2.18 and 1.41-1.95 for incisors and canines, respectively. Conclusion Although statistically less enamel colour changes occurred in the Kurasper F group compared with the Grengloo and Light Bond groups, the observed changes were not clinically relevant.