Research Journal of Biological Science, vol.13, no.2, pp.37-44, 2020 (Peer-Reviewed Journal)
The aim of this study was to investigate the isolation and typing of Nocardia species, which
are found in many water resources and have microbial importance. Agent isolations were carried out from
sediment samples taken from different points determined around Iğdır Fish Lake. DNA isolations were
carried out from the isolated Nocardia species. Subsequently, 16S rRNA gene region sequence analyzes
were performed. Sequence analyzes for each isolated bacteria were aligned and these results determined
similarities and differences at the level of group and genus of different origins and individual populations.
As a result of the study, 2 different types of bacteria were isolated. In the GenBank database, the isolated
bacteria were found to have 99.78% and 99.30% similarity with the species with 99% and higher sequence
matching. Nucleotide sequencing differences were found to be 0.3% with reported to be isolated from
different water sources. Different studies are needed to determine the differences at the gene level of the
same type of bacteria isolated from different sources, and to determine the pathogenicity and virulence
characteristics among the isolates.