Molecular analysis of oxa-48 producing k. pneumoniae strains isolated from patients with catheter-associated sepsis


Akgül Ö., Bora G.

European review for medical and pharmacological sciences, vol.26, no.20, pp.7724-7731, 2022 (SCI-Expanded) identifier identifier

Abstract

OBJECTIVE: K. pneumoniae is an important cause of hospital and community-acquired infections. In particular, carbapenem-resistant strains of K. pneumoniae spread globally, increasing the public health risk. This study aims to sequence and phylogenetically analyze K. pneumoniae strains isolated from blood cultures of patients in intensive care units in our hospital. MATERIALS AND METHODS: In this study, blood samples were collected from patients with catheter-related sepsis. Culture, biochemical, antibiotic susceptibility, and molecular tests were performed as microbiological analyses. RESULTS: Twenty-four K. pneumoniae strains showing multidrug resistance by isolating 276 K. pneumoniae were included in the study. It was determined that they showed the highest resistance against Ampicillin, Amoxicillin/ Clavulanic Acid, Ceftazidime, and Ceftriaxone. The comparison determined that K. pneumoniae isolates from different countries isolated from blood cultures had closeness and distance in OXA-48. CONCLUSIONS: After multilocus sequence typing, all of our 24 K. pneumoniae isolates were determined to be ST11.