1st International Veterinary Biochemistry and Clinical Biochemistry Congress (1.IVBCB-2018), Hatay, Turkey, 12 - 15 April 2018, vol.1, no.1, pp.124-125
Aim: This study was planned to evaluate the antigenotoxic potential of
thymoquinone (TQ) against DNA damage in NRK-52E cells treated with high
glucose by single cell gel electrophoresis (comet assay) technique.
Material and Methods: Cells were grown in vitro under regular conditions. The
cells were planted in the plates, 106
cells. Proliferative concentration of
thymoquinone (10μM) and IC25 (3rd hour: 550mM, 24th hr: 200mM) and IC50 (3rd
hour: 760mM, 24th hr: 280mM) values for each hour of glucose were determined
by MTT. Cells were incubated at these concentrations for 3 hours and 24 hours,
with control (K), thymoquinone (TQ), high glucose (G), high glucose and
thymoquinone (G + T) groups. DNA damage was assessed by the Comet assay
protocol and the results were calculated as genetic damage index (GDI).
Results: GDI levels were significantly higher (p <0.05) in all groups treated with
high glucose compared to control, a significant decrease was noted in all groups
with added thymoquinone. A significant increase (p <0.05) was also observed in
the thymoquinone group compared to the control group.
Conclusion: At high concentrations glucose was found to have genotoxic effects
in renal cells and TQ treatment with concentration and time-dependent high glucose
was found to be a significant effect in reducing DNA damage. However it was
concluded that only thymoquinone administration increased the GDI value
significantly compared to the control and that this was worth to be investigated in
Keywords: comet assay, genotoxicity, high glucose, NRK-52E, thymoquinone