The determination of the effect of thymoquinone on the DNA damage in the high glucose applied cell with the comet assay


Usta A., Dede S., Yüksek V., Öner A. C.

1st International Veterinary Biochemistry and Clinical Biochemistry Congress (1.IVBCB-2018), Hatay, Turkey, 12 - 15 April 2018, vol.1, no.1, pp.124-125

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Hatay
  • Country: Turkey
  • Page Numbers: pp.124-125

Abstract

Aim: This study was planned to evaluate the antigenotoxic potential of thymoquinone (TQ) against DNA damage in NRK-52E cells treated with high glucose by single cell gel electrophoresis (comet assay) technique. Material and Methods: Cells were grown in vitro under regular conditions. The cells were planted in the plates, 106 cells. Proliferative concentration of thymoquinone (10μM) and IC25 (3rd hour: 550mM, 24th hr: 200mM) and IC50 (3rd hour: 760mM, 24th hr: 280mM) values for each hour of glucose were determined by MTT. Cells were incubated at these concentrations for 3 hours and 24 hours, with control (K), thymoquinone (TQ), high glucose (G), high glucose and thymoquinone (G + T) groups. DNA damage was assessed by the Comet assay protocol and the results were calculated as genetic damage index (GDI). Results: GDI levels were significantly higher (p <0.05) in all groups treated with high glucose compared to control, a significant decrease was noted in all groups with added thymoquinone. A significant increase (p <0.05) was also observed in the thymoquinone group compared to the control group. Conclusion: At high concentrations glucose was found to have genotoxic effects in renal cells and TQ treatment with concentration and time-dependent high glucose was found to be a significant effect in reducing DNA damage. However it was concluded that only thymoquinone administration increased the GDI value significantly compared to the control and that this was worth to be investigated in further studies. Keywords: comet assay, genotoxicity, high glucose, NRK-52E, thymoquinone