AL-FARABI 4th INTERNATIONAL CONGRESS ON APPLIED SCIENCES, Erzurum, Turkey, 19 August - 21 September 2022, pp.343-350
Pyracantha coccinea Roem, known as fire thorn in our country. It is a perennial shrub-shaped plant of the Rosaceae family, with evergreen leaves, reaching a length of about three meters. The red and small fruits of Pyracantha are used in folk medicine due to their high antioxidant capacity, diuretic, cardiac and tonic properties. In this study, extracts of fire thistle plant prepared with different solvents (acetone, water, methanol, ethanol) manually and with an accelerated solvent extraction (ASE) device were compared in terms of antioxidant capacity (Cuprac; cupric reducing antioxidant capacity). After the dried and ground fire thorn fruits were extracted with different solvents at high temperature (80°C) and pressure (1500 psi) with the ASE device, the antioxidant activity was determined by using Cuprac method, As a manual method, the dried and ground plant was kept in solvent (ethanol, methanol, water, acetone) for 24 hours at room temperature and then centrifuged at 10000 rpm. After centrifugation, toxic solvent was removed by evaporation and Cuprac activity was determined. When both methods were compared, the Cuprac activity of the manual extraction with methanol solvent was found to be quite high. The lower Cuprac activities of the extracts obtained with the ASE device were associated with high temperature and pressure. When using the healing properties of a medicinal plant, its antioxidant capacity is expected to be at its maximum level. It is thought that various extraction methods should be investigated in order to use medicinal plants, which are less harmful than synthetic drugs, with higher efficiency and as active pharmaceutical ingredients.
Key words: Medicinal plant, Fire thorn, plant extraction, antioxidant capacity, Cuprac