The International Conference, Agriculture for Life, Life for Agriculture, Bükreş, Romania, 4 - 06 June 2015, pp.14
THE HAEMOGLOBIN, TRANSFERRIN, CERULOPLASMIN AND GLUTATHIONE POLYMORPHISM OF NATIVE GOAT BREEDS OF TURKEY, II- KILIS AND HONAMLI
Ayse Ozge DEMIR1, Nihat MERT2
1 University of Yuzuncu Yil, Faculty of Agriculture, Department of Animal Science, 65080, Van, Turkey, Phone: +90 538 654 61 61, Email: email@example.com
University of Yuzuncu Yil, Faculty of Veterinary Medicine, Department of Biochemistry, 65080, Van, Turkey, Phone: +90 532 376 13 18, Email: firstname.lastname@example.org
Corresponding author email: email@example.com
In this research, Kilis and Honamli goats are used, which are specific local genetic resources of Turkey. The herds were independent, but they had similar care and nutrition circumstances. From each breed 30 samples were taken, in all 120 samples were collected. Erythrocyte, whole blood and serum samples were used for haemoglobin (Hb), glutathione (GSH) and transferrin (Tf), ceruloplasmin (Cp) analysis, respectively. In the analysis of these samples, Hb and Tf bands were determined by electrophoresis. However, Cp and GSH levels were analyzed by the spectrophotometer. 3 Hb phenotypes (AA, BB, AB) and 6 Tf phenotypes (AA, AB, AC, BB, BC, CC) were determined in this study. In addition, both the observed and the expected values of polymorphic characteristic for 2 characters were presented according to the Hardy-Weinberg Equilibrium (HWE). Cp levels were detected as 0.822 ± 0.055 mg/dl and 1.793 ± 0.109 mg/dl in Kilis and Honamli herds, respectively. Also GSH levels were detected as, 42,486 ± 1,034 mg/dl and 33.515 ± 0.345 mg/dl in these breeds, respectively. On the other hand, the high and low GSH levels (GSHH and GSHh) of herds were presented.
Key words: electrophoresis, gene resource, goat, spectrophotometer.