In this study, nephrotoxic effect of Amphotericin B (AmB) was investigated together with clinical, biochemical, and histopathological findings in dogs intoxicated with different doses and methods of administration. 18 healthy cross-bred dogs were allocated to three groups. Amphotericin B diluted with sterile water was used. Dogs in group A (n = 6) were treated with 0.5 mg/kg AmB in 25 ml 5% dextrose as a rapid bolus (4-5 min). Dogs in group B (n = 6) were treated with 1 mg/kg AmB in 50 ml 5% dextrose as a rapid bolus (4-5 min). Dogs in group C (n = 6) were treated with 2 mg/kg AmB in 1000 ml 5% dextrose as a slow infusion (4-5 h). Clinical, haematological and biochemical analyses were made in all dogs before the experiment as well as on the 5(th) and 12(th) days. Haematological and biochemical values recorded before the experiment were compared with those on the 5(th) and 12(th) day.