Hepatoprotective effect of diffractaic acid on carbon tetrachloride-induced liver damage in rats


Karagoz I. D. , Ozaslan M., Kilic I. H. , Guler I., Uyar C., Tuter D., ...Daha Fazla

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.29, ss.1011-1016, 2015 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 29 Konu: 5
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1080/13102818.2015.1056754
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Sayfa Sayıları: ss.1011-1016

Özet

This study was designed to investigate the hepatoprotective effect of diffractaic acid isolated from a lichen species, Usnea longissima, at 3 doses, 50, 100 and 200 mg/kg, against carbon tetrachloride (CCl4)-induced hepatic damage. For this purpose, 40 Wistar albino rats were divided in 5 groups, including 3 experimental and 2 control ones, and 0.2 mL/kg of CCl4 in olive oil (1:5 v/v) was injected daily for 6 weeks intraperitoneally. After the liver injury, diffractaic acid was applied at doses of 50, 100 and 200 mg/kg for 7 days. The group given physiologic saline (0.2 mL/kg) was used as a control group. Alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, creatinine, urea, direct and total bilirubin and C-reactive protein levels were also evaluated in the serum samples obtained from the rat groups. Liver tissues were removed and were examined histopathologically following staining with hematoxylin-eosin. The results showed that 50 mg/kg daily dose of diffractaic acid could be considered to have hepatoprotective effect by ameliorating the studied biochemical parameters and tissue histological structures. However, 100 and 200 mg/kg of diffractaic acid acted as hepatotoxin and did not show any hepatoprotective effect. Thus, diffractaic acid could be potentially used as a hepatoprotective agent at a low dose (50 mg/kg) against acute liver toxicity induced by CCl4.