In this study, optimization of medium conditions for CBY2 adsorption by pumice was studied through experimental design. For CBY2 adsorption, initial CBY2 concentration (C-o, mg/L), pH and contact time (min.) were determined as medium conditions. Central Composite Design (CCD) within response surface methodology (RSM) was applied to develop a response surface for optimization of adsorption conditions. The optimum conditions were determined as C-o = 154.28 mg/L, pH = 6.13 and contact time = 47.98 min. Results of experiments determined that at these optimum conditions, optimum adsorbed amount was 12.62 mg/g and adsorption yield was 81.79%. A quadratic model was developed by CCD to represent CBY2 adsorption.