Effect of Bisphenol-A on Oxidative Status and Certain Inflammatory Markers in Rats: Experimental Study

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Çibuk S., Yılmaz H. C., Mert H., Mis L., Yörük M., Mert N.

Türkiye klinikleri veteriner bilimleri (online), vol.14, no.2, pp.39-46, 2023 (Peer-Reviewed Journal)


Bisphenol-A (BPA) is a public health concern due to its widespread use in the living world and consumer products. It aimed to study the effect of ingested BPA on oxidative status and certain inflammatory markers in rats. Material and Methods: In this study, 24 Wistar Albino rats were used, and divided into 4 groups (control, sham, BPA25, BPA50). While no application was made to the first of these groups, corn oil was given to the second group (sham). In the first of the experimental groups, BPA dissolved in corn oil was administered at 25 mg/kg/day, and in the second at 50 mg/kg/day by oral gavage for one month. At the end of the experiment, the animals were anesthetized. Blood samples were taken from the heart and the sera were separated. Total antioxidant capacity, the total oxidative state, tumor necrosis factor-alpha, interleukin (IL)-1 beta, and IL -6 levels were determined by enzyme-linked immunosorbent assay. Results: When BPA was administered in doses of 25-50 mg/kg/day, serum total antioxidant capacity levels significantly decreased, while an increase was seen in total oxidative status and Oxidative Stress Index reflects harmful effects of BPA. Furthermore, serum tumor necrosis factor-alpha, IL-1-beta, and IL-6 levels increased significantly (p≤0.05). Conclusion: These results suggest that BPA exposure induces inflammatory markers. BPA also may causes oxidative stress, not only by promoting the production of oxidative compounds but also by reducing antioxidant capacity compared to the control group. All these biochemical changes may lead to damage to macromolecules in the tissues of living organisms.