DETERMINATION OF THE ANTIBACTERIAL EFFECT OF BEE VENOM AGAINST RAINBOW TROUT PATHOGENS AND ANTIBIOTIC RESISTANCE GENE EXPRESSION

Kabakcı D., Ürkü Atanasov Ç., Önalan Ş.

ACTA VETERINARIA, cilt.73, sa.3, ss.374-388, 2023 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73 Sayı: 3
  • Basım Tarihi: 2023
  • Dergi Adı: ACTA VETERINARIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), EMBASE, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.374-388
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

L.). It contains a variety of bioactive ingredients including peptides, proteins, enzymes,

and volatile metabolites. This study investigated the antibacterial effects of the bee

venom obtained from honey bees (Apis mellifera L.) against bacterial fish pathogens,

such as Lactococcus garvieae (Lg1, Lg2, Lg3), Vibrio anguillarum (Va1, Va2, Va3), Yersinia

ruckeri (Yr1, Yr2, Yr3), and Aeromonas hydrophila (Ah1, Ah2, Ah3) and the expression

levels on the antibiotic resistance genes hly and fbp (hemolysin and fibronectin-binding prot)

of them. It was determined that bee venom had an antibacterial effect against L. garvieae,

L. anguillarum, and Y. ruckeri strains, while it had no effect only against Ah3 and Ah2


bacterial strains. As staded by the gene expression of hly (hemolysin) and fbp (fibronectin-

binding protein), among the antibiotic resistance genes the effect levels of bee venom on


bacterial species varied, although it affected antibiotic resistance and gene expression

level in all bacteria. It was revealed that the expression level was the highest for V.

anguillarum strains, whereas it was below the control group for L. garvieae. i.e the effect

of bee venom on the resistance mechanism for L. garvieae was much less compared to V.

anguillarum. Based on the results in the current study it could be concluded that applying

bee venom to pathogenic bacteria that cause mortality in the aquaculture sector could

induce the defense-related gene and change the broad-spectrum biocontrol activity at

the molecular level.