Akademik Veri Yönetim Sistemi
International Biochemistry Congress – 2017 /28 th National Biochemistry Congress (TurkJBiochem-2017), Erzurum, Türkiye, 19 - 23 Eylül 2017, cilt.42, sa.1, ss.41
OBJECTIVES: This study was planned to determine the effect of certain vitamin
applications on total antioxidant capacity (TAS),total oxidant capacity (TOS) and
to evaluate the antioxidant role of vitaminsC and E against possible toxic effects
of fluoride ,in renal cells exposed to sodium fluoride in vitro .
MATERIALS- METHODS: Cells were grown in regular conditions with in vitro
conditions . Cells were plated into plates,1x106 cells.Cells; at various doses
NaF(100-10000 μM), 60μM vitaminE , 100μM vitaminC , sodium fluoride and
vitamin groups were given together as a control group and were separated.MTT%
viability results were determined.as the control group was accepted as 100% live.
Twenty-four hour incubation followed by trypsinized cells were prepared with
broken up freeze /thaw method and analyzed .Twentyfour hour incubation
followed by trypsinized cells were dissociated by freeze /thaw method and
analyzed .In the obtained cell lysate ; TAS and TOS values were determined by
ELISA method using commercial kit .
RESULTS : TAS levels in all groups were found to decrease significantly in the
groups given only vitamin and NaF+vitamins(p≤0.05).It was observed that TOS
levels in all groups increased significantly from control(p≤0.05).For OSI, it
was observed that all groups increased significantly in relation to the control, the
use of vitaminE in combination with NaF increased the OSI and there was no
significant change in the group given only NaF + vitamin C .
CONCLUSIONS: In conclusion,although the NRK-52E rat kidney cell line has a
decreasing effect on the cell viability of the NaF dose, the changes in the TAS/TOS balance and the effect of NaF and vitamin in the applied groups were
found to be limited in terms of the mechanisms of cell death.
Keywords: Fluoride, cell culture, total antioxidant capacity, total oxidant capacity,
vitamin