The purpose of this study was to evaluate genetic similarities and distances among pure lines of poultry using the RAPID technique. In this study four layer and three meat type pure lines were used. Verous blood samples from five males and five females of each line were collected from the venae cutenea ulnaris. The RAPID technique was used to generate a DNA fingerprint of lines. Initially, a total of 35 primers having arbitrary sequences were used for RAPID analysis. The amplicons were resolved on 1.5% agarose gel and stained with ethidium bromide. To evaluate the bands, polymorphic and monomorphic bands were described. Genetic similarities and genetic distances were calculated in meat and layer pure lines. The RAPID analysis data from 23 primers were utilized in estimating genetic similarities, which ranged from 0.644 to 0.853 between the lines. The maximum genetic distance was observed between the L2 meat dam line and L4 layer sire line (0.440).