Determination of essential oil and biological activities of Hypericum ternatum Poulter and H. scabrum L. species collected from different localities: is H. scabrum an alternative to multifunctional species ST JOHN’S WORT (H. perforatum)?

Akdeniz M., Yener I., İrtegün S., Özhan Kocakaya Ş., Fırat M., Yiğitkan S., ...More

TURKISH JOURNAL OF CHEMISTRY, vol.46, no.1, pp.1956-1971, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 46 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.55730/1300-0527.3494
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1956-1971
  • Van Yüzüncü Yıl University Affiliated: Yes


The importance of Hypericum L. species, being used in traditional medicine, in the scientific world is increasing day by day.

Hypericum species are plants that have been used in the treatment of many diseases for a long time and have healing properties. In

the current study, the essential oil compositions of Hypericum scabrum L. and H. ternatum Poulter collected from different localities

in Turkey were determined by GC-MS/FID. In addition, their antioxidant, cytotoxic, and antimicrobial activities with their enzyme

inhibitory potentials (cholinesterase, urease, tyrosinase, elastase, and collagenase) were investigated. Also, in vitro and in silico studies

of the major components of the species have been carried out on the studied enzymes. It was determined that four H. scabrum samples

mainly contained α-pinene (55.99%–62.80%) while three H. ternatum samples contained 2-methyloctane (9.45%–22.39%) and α-pinene

(12.75%–33.08%). While H. scabrum essential oils possessed significant biological activity potential except for the antimicrobial activity,

H. ternatum samples did not. All essential oil samples of H. scabrum exhibited a high cytotoxic effect (IC50 21.67 ± 0.34 to 34.67 ± 0

.45 μg/mL) against colon cancer cell line (HT-29) and indicated higher acetyl- (Inhibiton% 83.30 ± 1.90 to 93.08 ± 1.04) and butyrylcholinesterase

(Inhibiton% 80.58 ± 1.19 to 93.28 ± 1.99) inhibitory activity than the standard compound, galantamine. Furthermore,

all samples of H. scabrum showed high tyrosinase (Inhibiton% 69.00 ± 1.64 to 95.25 ± 1.42) and elastase (Inhibiton% 27.58 ± 0.34 to

36.69 ± 0.18) inhibitory effects. These biological activity results indicated that H. scabrum essential oil could be used in the cosmetic and

pharmaceutical industries.