© 2022, The Author(s), under exclusive licence to Der/die Autor(en), exklusiv lizenziert an Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature.The study was carried out to determine the pomological, phytochemical and antimicrobial properties of wild raspberry fruit (Rubus idaeus) naturally growing in the Kelkit Valley in Turkey. In the study, the wild raspberry fruit was comparatively smaller. In fruit, soluble solids content (SSC) was determined as 12.6%, titratable acidity as 1.36% and vitamin C concentration as 28.8 mg 100 g−1. Total phenolics, total flavonoids, 1,1-diphenyl-2-picrylhydrazil (DPPH) and ferric ions (Fe+3) reducing antioxidant power assay (FRAP) activity values were 1775 mg kg−1, 151 mg kg−1, 2580 µmol kg−1 and 5187 µmol kg−1, respectively. In the study, the solution obtained from raspberries had an antimicrobial effect on bacteria. While it had an antimicrobial effect on Aspergillus niger fungus, it did not have any effect on Candida albicans fungus. The highest antimicrobial activity was achieved against Pseudomonas aeruginosa, while the lowest effect was against Enterococcus feacalis bacteria. It was revealed that the raspberry fruit investigated in the study can be used as material for breeding studies due to their rich bioactive compounds and antimicrobial content.