Exploring thermodynamics and kinetic parameters of immobilized catalase enzyme via adsorption on krill clay


Nas M. S. , Gur A. , Gür T. , Yonten V.

DESALINATION AND WATER TREATMENT, cilt.67, ss.178-186, 2017 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 67
  • Basım Tarihi: 2017
  • Doi Numarası: 10.5004/dwt.2017.20389
  • Dergi Adı: DESALINATION AND WATER TREATMENT
  • Sayfa Sayıları: ss.178-186

Özet

The thermodynamic parameters and immobilization kinetics of catalase enzyme via adsorption onto krill clay were performed in aqueous solution in batch system. The adsorbent was characterized by X-ray fluorescence, scanning electron microscope and Brunauer-Emmett-Teller N-2 adsorption measurements. The effect of some parameters to the adsorption process such as contact time, stirring speed, initial enzyme concentration, initial ionic strength, temperature and pH were analyzed. Maximum adsorption capacity (q(m)) showed a great dependence on pH and initial enzyme concentration. It was found at pH 5.5, and initial enzyme concentration, 0.0075 g L-1 values. Pseudo-first-order, pseudo-second-order and intraparticle diffusion were used to fit the kinetics data on kinetic models. Furthermore, thermodynamic activation parameters such as enthalpy, entropy, Gibbs free energy and activation energy (Delta H degrees, Delta S degrees, Delta G degrees and E-a) which reveal that catalase enzyme adsorption occur spontaneously and in endothermic nature were determined. The obtained results confirmed that the applicability of krill clay is an efficient immobilizing supporter for catalase enzyme and krill clay material can be used as a very effective adsorbent in immobilization of this enzyme from aqueous solutions.