Cytotoxic activity of wild apple seed (Malus sylvestris Miller) extracts on HepG2 cells with their antioxidant properties

Eray Vuran N., Irtem Kartal D., Çelik I.

4th International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2022), Ankara, Turkey, 14 - 16 December 2022, pp.34

  • Publication Type: Conference Paper / Summary Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.34
  • Van Yüzüncü Yıl University Affiliated: Yes


This study was designed to investigate the in vitro antioxidant and antiproliferative activities of the ethanolic and water extracts of wild apple seed (WAS) (Malus sylvestris Miller subsp. orientalis). Antioxidant potentials of the extracts were evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging capacity tests. Antiproliferative activities of the extracts were tested against HepG2 (hepatocellular carcinoma) cell lines with XTT test. As a result, IC50 values for DPPH assay were determined as 45.8±0.12, 6.80±0.14 mg/mL for WAS water and ethanolic extracts, respectively. The antioxidant activity of WAS ethanolic extracts were calculated as 5.04±0.03 µg TEAC/mg extract for ABTS+ assay. WAS ethanolic extracts displayed 1.18-fold more antiproliferative activity than the water extract against HepG2 cells. The IC50 values of the ethanolic and water extracts of WAS were determined as 4.56 and 3.87 mg/mL, respectively. As a result, the ethanolic extracts of WAS showed the higher antioxidant and antiproliferative activity than the water extracts. Wild apple seeds have cytotoxic activity against HepG2 cell line and may be use as a therapeutic agent in the treatment of cancer.