Akademik Veri Yönetim Sistemi
Black Sea Journal of Agriculture, cilt.9, sa.1, ss.89-99, 2026 (TRDizin)
In this study, the effects of different doses of ascorbic acid (AA) applications on the physiological and biochemical responses of medicinal sage under salt stress were evaluated in a multifaceted manner. In the study, ascorbic acid was applied at different concentrations (0 ppm, 100 ppm, 200 ppm, and 300 ppm), and the plants were exposed to salt stress at different doses (0 mM, 100 mM,200 mM and 300 mM NaCl). In the study, Dualex parameters (NBI (Nitrogen Balance Index), chlorophyll, flavonoids, anthocyanins) and some biochemical components (total antioxidant activity, total flavonoids, total phenolics, and ascorbic acid content) were determined. Based on the obtained data, the total ascorbic acid content was found to be statistically insignificant, while all other parameters were found to be significant. The lowest and highest values for biochemical parameters were total antioxidant activity 68.78-176. 59 μmol TE g-1, total flavonoids 6.48-13.77 mg QE 100g-1, total phenolic content 174.00-237.39 mg GAE g-1, and ascorbic acid 19.69-24.92 mg 100g-1. In Dualex measurements, the lowest and highest values were found to be 37.50–74.70 dx for NBI, 25.83–49.40 dx for chlorophyll, 0.56–0.87 dx for flavonoids, and 0.013–0.080 dx for anthocyanins. The findings indicate that both AA doses and salt stress levels, either alone or in combination, cause significant changes in the plant's defense mechanisms and metabolic regulation.