Evaluation Of Biochemical Parameters Of Laccase Enzyme Immobilized On Various Clay Minerals According To Free Enzyme

Özdemir F., Yalçınkaya Z.

4.EBAT EURASIA BIOCHEMICAL APPROACHES&TECHNOLOGYIES, Antalya, Turkey, 3 - 06 November 2022, pp.115

  • Publication Type: Conference Paper / Summary Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.115
  • Van Yüzüncü Yıl University Affiliated: Yes


In this study, the immobilization of the laccase enzyme, which has a wide application area in the industry, was examined. For this purpose, commercially obtained laccase enzyme was immobilized on various clays using the physical adsorption method. The effects of pH, temperature, substrate concentration, and storage time on the activity of free and immobilized lacquers were examined. As a result of the studies, the optimum pH and temperature for free laccase were obtained as 5.5 and 40 °C respectively and the optimum pH and temperature for all immobilized enzymes (bentonite, diatomite, and Bardakçı) were 5.5-6.0 and 40 °C respectively. The effects of pH and temperature on the activity of the immobilized laccase showed that the properties of the immobilized enzyme were the same as those of the free enzyme. The resulting kinetic constant values turned out to be quite close to each other. In addition, it was shown that adsorption did not significantly affect the kinetic properties of the enzyme. Only 20%-30% of immobilized laccase activity disappeared in 2 months. KM values for free enzyme and immobilized enzymes were found as 0.0700 mM, 0.0724 mM, 0.0831 mM and 0.0935 mM and Vmax values were 0.0695, 0.0216, 0.0236 and 0.0233 mM-1, respectively. The KM value of the immobilized enzyme is greater than that of the free enzyme and the Vmax value is smaller. The increase in resistance of the immobilized enzyme to temperature change and storage time indicates that laccase immobilization on clay is beneficial for enzyme immobilization.