Ticks Rhipicephalus sanguineus of Rhipicephalus strain of Ixodidae family are biological mediators to transfer of Rickettsia pathogens such as Ehrlichia and Anaplasma in animals In the present study, a total of 220 ticks were collected from 55 infested dogs. The ticks' taxonomy was carried out through microscopically. Additionally, DNA extraction from ticks was conducted and nested PCR was performed to differentiate the Ehrlischia spp. The obtained results indicates that collected ticks were belong to Rhipicephalus sanguineus of Rhipicephalus strain of Ixodidae family of ticks. The PCR results showed Ehrlichia spp. was present in ticks and E. canis was more common (50 of 220 ticks) than E. ewingii (3 of 220 ticks). In conclusion, E. canis was firstly detected in R. sanguineus tick in dogs in the Province of Van in Turkey. E. ewingii was firstly detected in R. sanguineus tick in dogs in Turkey. Its presence in dogs indicates the potential factor for public health hazard in Turkey.