Protective Effects of Chrysin in Rats with Ovarian Torsion


Dirik D., Kömüroğlü A. U., Koşal V., Başbuğan Y., Özdek U., Kolusari P., ...More

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.27, no.4, pp.417-424, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.9775/kvfd.2021.25357
  • Journal Name: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.417-424
  • Keywords: Ovary torsion, Chrysin, 8-OHdG, Antioxidant, GELATINASE-ASSOCIATED LIPOCALIN, ISCHEMIA-REPERFUSION INJURY, ACUTE KIDNEY INJURY, OXIDATIVE STRESS, ISCHEMIA/REPERFUSION INJURY, MODEL, INTERLEUKIN-18, FLAVONOIDS, DETORSION, TORSION/DETORSION
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

The objective of this study is to assess the protective effect of chrysin (CH) against ovarian torsion-detorsion injury. Thirty-two female albino rats were divided into 4 groups as Control, Torsion/Detorsion (T/D), TD-CH, and CH. Ovarian torsion was done for 3 hours on TD and TD-CH groups and then detorsion was performed. 50 mg/kg of CH was administered to the TD-CH group via oral gavage half an hour before the detorsion. Subsequently, 50 mg/kg of CH was administered via oral gavage to TD-CH and CH groups for 14 days. At the end of the experiment, blood samples were collected and ovarian tissue were taken. SOD and GPx activities and GSH and 8-OHdG levels were studied in serum and ovarian tissue. Also, IL-18, KIM-1, NGAL, Cys-C levels were studied in serum samples. GSH levels and GPx, SOD activities in both serum and ovarian tissue were significantly lower in TD group compared to the control and TD-CH groups (P<0.05), whereas the 8-OHdG level was significantly higher (P<0.05). Treatment with CH resulted in a decrease in 8-OHdG level and an increase in GSH level, GPx and SOD activities in both serum and ovarian tissue in the TD-CH group compared to TD group (P<0.05). Chrysin could ameliorate ovarian injury. Essentially, this outcome is thanks to the antioxidant, anti-inflammatory, and antiapoptotic effects of chrysin. Furthermore, it also has significant effects on DNA oxidative damage.