Akademik Veri Yönetim Sistemi
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, cilt.66, ss.1-14, 2023 (SCI-Expanded)
Drought stress, which is becoming more frequent and severe, restricts grain production. It is critical to choose wheat (Triticum aestivum
L.) cultivars that are drought-resistant enough in progressively arid
climates. Early physiological and biochemical responses were measured by
applying drought stress to susceptible Bezostaja-1 and tolerant
Tosunbey wheat cultivars with three different PEG-6000 concentrations to
understand the reliability, selectivity and practicality of tested
parameters. Depending on the elevated drought, lengths, fresh-dry-turgor
weights, relative water content of both root and stem decreased, and
chloropyll amounts increased similarly but in different dimensions in
both cultivars. While a decrease was observed in the total thiol content
of both, it was determined that this value was 10 times higher in
Bezostaja-1 cultivar. While proline amounts peaked at the most severe
drought, this value was found to be 2.5 times higher in Bezostaja-1 than
in Tosunbey. The protein content is 35% higher in Bezostaja-1. In
contrast to glutathione S-transferase theta1 (GSTT1) isozyme, total GST
activity increased 53.1% in Bezostaja-1 and 33.6% in Tosunbey, compared
to control. This average values are about three times higher in
Bezostaja-1 compared to Tosunbey. Of the experimental groups whose band
profiles were generated by SDS-PAGE and compared with UPGMA and
neighbor-joining methods, moderate to high drought samples of
Bezostaja-1 showed explicitly different results and possibly a
metabolically distinct response from all others. Besides frequently used
ones, total thiol content, total GST activity and changes in the
protein bands could be used as useful parameters in the selection of
drought-resistant cultivars.