In biomedical applications, silver nanoparticles (Ag NPs) are of great interest due to their cost-effective and environmentally friendly properties. Green synthesis of nanoparticles for biological research is a preferred choice since it does not require additional reducing agent. For this purpose, in this study, we aimed to synthesize the biogenic silver nanoparticles with the help of Rosa canina plant (Rc-Ag NPs) and then they have been tried for their antioxidant and antibacterial properties. UV-Vis spectrophotometer, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) analyses were performed for characterization of Rc-Ag NPs. Antioxidant properties of silver nanoparticles synthesized with Rosa canina plant were investigated against 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH). DNA dissociation activity of synthesized Rc-Ag NPs was studied, and DNA dissociation activity was shown. The antimicrobial activity of Rc-Ag NPs was also tested using micro-dilution. According to the results, Rc-Ag NPs synthesized were found to be highly effective for anti-oxidant, antibacterial, antifungal, and DNA cleavage activities.