Detection of Arbuscular Mycorrhizal Fungi Within Colonized Roots of The Gramineae Family Members by Nested-PCR

Demir S., Sıpahıoglu H. M., Kaya İ., Usta M., Savur O. B.

Archives of Phytopathology & Plant Protection, vol.44, no.1, pp.1061-1067, 2011 (Peer-Reviewed Journal)


In this study, it is aimed to asses the association of arbuscular mycorrhizal (AM)
fungi within colonised rhizosphere of Gramineae family members through a
survey by using nested- polymerase chain reaction method in Van province
(Turkey). From 24 agro-ecological fields, a total of 82 samples belonging to
Gramineae family were tested by molecular methods. The presence of
and Glomus mosseae was ascertained in 10 plants belonging to eight
different species by using fungus specific primers. Root colonisation ranged from
6 to 37% within rhizosphere of Gramineae family members and the average root
colonisation by AM fungi was 22%.