The effects of replacing corn in the diets of laying hens with 100% barley containing different enzymes as an energy source on different yields and egg quality traits were investigated in this study. A total of 300 laying hens (average 22 weeks of age) were used. The study was carried out with five groups, 2 controls (60% corn, 60% barley) and 3 experimental groups. Each group of 60 hens was also divided into six subgroups. Enzymes such as Betaglucanase (Allzyme BG), xylanase (Allzyme PT) and amylase (Allzyme AB) were separately added at a rate of 0.05% in the order of betaglucanase, betaglucanase + xylanase and betaglucanase + xylanase + amylase to each of the groups fed 60% barley.