The effect of lycopene onto apoptotic pathway caused by NaF


Yüksek V., Çetin S., Usta A., Dede S., Taşpınar M.

2.Medicine and Health Sciences Researches Congress (2.UTSAK-2019), Şanlıurfa, Turkey, 1 - 03 November 2019, vol.1, no.11, pp.473-481

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Şanlıurfa
  • Country: Turkey
  • Page Numbers: pp.473-481
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Abstract Objectives: In this study, it was aimed to investigate therapeutic effect of lycopene (LYC) on the apoptosis induced through the sodium fluoride (NaF) in the NRK-52E cell line. Materials and Methods: The cytotoxic effect of NaF and Lycopene in NRK-52E renal epithelial cells was determined by MTT method. Cytotoxic (for IC50, NaF) and proliferative (for LYC) concentrations of molecules were administered to Control, NaF, LYC and NaF+LYC formed into 4 groups and then the expression of the genes in the apoptotic pathway was determined by RT-PCR. Result: The IC50 value of NaF was determined to be 3200µM for 24 hours, while no cytotoxicity was detected for LYC. LYC showed the highest proliferation effect at 1µM concentration in 24 hours. Therefore, the apoptotic effects of the molecules have been selected concentration IC50 for NaF and 1 µM for LYC. In the NaF+LYC group, caspase 8 expression increased 4.4, caspase 3 increased 14-fold and Bcl-2 gene increased 4.6-fold, while no significant change was observed in the other groups. Conclusion: In this study, it was determined that the application of NaF together with LYC in kidney cells may decrease the expression of genes in mitochondrial pathway. As a result, it was determined that LYC administration in NRK-52E cells could increase NaF-induced apoptosis via receptor pathway. Keywords: Nrk-52e Cell Line, Naf, Lycopene, Apoptosis