Akademik Veri Yönetim Sistemi
2.Medicine and Health Sciences Researches Congress (2.UTSAK-2019), Şanlıurfa, Türkiye, 1 - 03 Kasım 2019, cilt.1, sa.11, ss.473-481
Abstract
Objectives: In this study, it was aimed to investigate therapeutic effect of lycopene
(LYC) on the apoptosis induced through the sodium fluoride (NaF) in the NRK-52E cell line. Materials and Methods: The cytotoxic effect of NaF and Lycopene in
NRK-52E renal epithelial cells was determined by MTT method. Cytotoxic (for
IC50, NaF) and proliferative (for LYC) concentrations of molecules were
administered to Control, NaF, LYC and NaF+LYC formed into 4 groups and then
the expression of the genes in the apoptotic pathway was determined by RT-PCR.
Result: The IC50 value of NaF was determined to be 3200µM for 24 hours, while
no cytotoxicity was detected for LYC. LYC showed the highest proliferation effect
at 1µM concentration in 24 hours. Therefore, the apoptotic effects of the molecules
have been selected concentration IC50 for NaF and 1 µM for LYC. In the
NaF+LYC group, caspase 8 expression increased 4.4, caspase 3 increased 14-fold
and Bcl-2 gene increased 4.6-fold, while no significant change was observed in the
other groups. Conclusion: In this study, it was determined that the application of
NaF together with LYC in kidney cells may decrease the expression of genes in
mitochondrial pathway. As a result, it was determined that LYC administration in
NRK-52E cells could increase NaF-induced apoptosis via receptor pathway.
Keywords: Nrk-52e Cell Line, Naf, Lycopene, Apoptosis