Assessment of 8-hydroxy-2-deoxyguanosine activity, gene expression and antioxidant enzyme activity on rainbow trout (Oncorhynchus mykiss) tissues exposed to biopesticide

ALAK G., UÇAR A., PARLAK V., Yeltekin A. Ç., Tas I. H., Olmez D., ...More

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, vol.203, pp.51-58, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 203
  • Publication Date: 2017
  • Doi Number: 10.1016/j.cbpc.2017.10.007
  • Journal Name: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.51-58
  • Keywords: Biopesticide, Fish, Oxidative stress, 8-OHdG, CYP1A, HSP70, OXIDATIVE DNA-DAMAGE, LIPID-PEROXIDATION, DELTAMETHRIN, STRESS, HSP70, LIVER, CHLORPYRIFOS, TOXICITY, IMPACT, CYP1A
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

The goal of this study was to determinate toxicity mechanism of biopesticide with antioxidant enzymes parameters such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and malondialdehyde (MDA) levels, oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)), transcriptional changes of heat shock protein 70 (HSP70), and cytochromes P4501A (CYP1A), sod, cat, and gpx in liver and gill tissues of Oncorhynchus mykiss. For this aim, plant-based (natural pesticides, azadirachtin (AZA)) and synthetic pesticides (deltamethrin (DLM)) were exposed on the fish at different concentrations (0.0005 and 0.00025 ppm of DLM; 0.24 and 0.12 ppm of AZA) for 21 days.