Assessment of 8-hydroxy-2-deoxyguanosine activity, gene expression and antioxidant enzyme activity on rainbow trout (Oncorhynchus mykiss) tissues exposed to biopesticide

ALAK G., UÇAR A., PARLAK V., Yeltekin A. Ç., Tas I. H., Olmez D., ...Daha Fazla

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, cilt.203, ss.51-58, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 203
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.cbpc.2017.10.007
  • Dergi Adı: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.51-58
  • Anahtar Kelimeler: Biopesticide, Fish, Oxidative stress, 8-OHdG, CYP1A, HSP70, OXIDATIVE DNA-DAMAGE, LIPID-PEROXIDATION, DELTAMETHRIN, STRESS, HSP70, LIVER, CHLORPYRIFOS, TOXICITY, IMPACT, CYP1A
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

The goal of this study was to determinate toxicity mechanism of biopesticide with antioxidant enzymes parameters such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and malondialdehyde (MDA) levels, oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)), transcriptional changes of heat shock protein 70 (HSP70), and cytochromes P4501A (CYP1A), sod, cat, and gpx in liver and gill tissues of Oncorhynchus mykiss. For this aim, plant-based (natural pesticides, azadirachtin (AZA)) and synthetic pesticides (deltamethrin (DLM)) were exposed on the fish at different concentrations (0.0005 and 0.00025 ppm of DLM; 0.24 and 0.12 ppm of AZA) for 21 days.