Novel Fluorine Boron Hybrid Complex as Potential Antiproliferative Drugs on Colorectal Cancer Cell Line

Tülüce Y., Masseh H. D. I., Koyuncu İ., Kılıç A., Durgun M., Özkol H.

ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, vol.19, no.5, pp.627-637, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 5
  • Publication Date: 2019
  • Doi Number: 10.2174/1871520619666190117142353
  • Journal Name: ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.627-637
  • Keywords: Antiproliferative, apoptosis, colorectal cancer cytotoxicity, DNA injury, chemotherapy, ELISA, IN-VITRO, APOPTOSIS, PROLIFERATION, GENERATION, INHIBITOR, POLYPOSIS, COMPOUND, TOXICITY, GENETICS, BREAST
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Objective: Colorectal Cancer (CRC) is one of the most common types of cancer in both sexes; it is considered to be the third leading death factor among other types of cancer.

 This study aimed to examine the cytotoxicity of a new fluorine boron hybrid complex [L(BF2)2] on human colorectal adenocarcinoma cell line (HT-29), based on the potency of the half-metal based complexes to initiate apoptosis.

 Methods: Based on this data, the impact of it in different concentrations on HT-29 cancerous cells was determined by apoptosis (ELISA, DNA fragmentation laddering, AO/EB staining), cytotoxicity (MTT) and genotoxicity (comet assay). We also calculated the cellular Oxidative Stress Index (OSI) by measuring the Total Antioxidant Status (TAS) and Total Oxidant Status (TOS).

 Results: Firstly, [L(BF2)2] was examined in view of cytotoxic effect in seven various cell lines (HELA, DU-145, PC3, DLD-1, ECC, PNT1-A and HT-29), and then it was found that the applied complex had a mighty antiproliferative action on HT-29 cells. Thus, the most effective IC50 value turned out to be 26.49 µM in HT-29 cell line. The present study found a tremendous efficacy of [L(BF2)2] on HT-29 cells, especially in terms of damage to cancer cells' DNA, and consequently caused a series of reactions leading to programmed cell death.

 Conclusion: The results suggest that the [L(BF2)2] as a novel fluorine boron hybrid complex can induce the apoptosis of HT-29 colorectal cancerous cell line and is a possible candidate for future cancer studies. 

Keywords: Antiproliferative, apoptosis, colorectal cancer cytotoxicity, DNA injury, chemotherapy, ELISA.