Comparison of microscopy, ELISA, and real-time PCR for detection of Giardia intestinalis in human stool specimens

Beyhan Y. E., Cengiz Z. T.

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.47, no.4, pp.1295-1299, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.3906/sag-1612-71
  • Journal Name: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1295-1299
  • Keywords: Giardia, detection, ELISA, real-time PCR, comparison, LINKED-IMMUNOSORBENT-ASSAY, LAMBLIA ANTIGEN, ENTAMOEBA-HISTOLYTICA, ENZYME IMMUNOASSAYS, FECAL SPECIMENS, SAMPLES
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens.