Atıf İçin Kopyala
Gülaydın Ö., Öztürk C., Ekin İ. H., İlhan Z., İlhan F.
ACTA VETERINARIA BRNO, cilt.92, sa.1, ss.69-77, 2023 (SCI-Expanded)
-
Yayın Türü:
Makale / Tam Makale
-
Cilt numarası:
92
Sayı:
1
-
Basım Tarihi:
2023
-
Doi Numarası:
10.2754/avb202392010069
-
Dergi Adı:
ACTA VETERINARIA BRNO
-
Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, Veterinary Science Database
-
Sayfa Sayıları:
ss.69-77
-
Anahtar Kelimeler:
Ovine, ruminants, reproduction, immunohistochemistry
-
Van Yüzüncü Yıl Üniversitesi Adresli:
Evet
Özet
Abortion causes significant economic losses in the sheep industry. Determination of the aetiology is important in dealing with abortions. The present study was aimed to identify selected important bacterial pathogens in the abortion cases of sheep. A total of 113 samples (105 aborted sheep foetuses, 4 placentas, and 4 vaginal swab samples) from 85 different sheep flocks were examined by real-time PCR (RT-PCR) regarding Chlamydia (C.) spp., C. abortus, Brucella (B.) spp., B. melitensis, Salmonella (S.) spp., S. Abortusovis, Coxiella (C.) burnetii, Listeria (L.) spp., L. monocytogenes, and Campylobacter spp. All cases that were found to be positive for bacterial agents by RT-PCR, were examined pathologically. Tissue samples of foetuses that were found to be positive for B. melitensis and L. monocytogenes by RT-PCR were also investigated immunohistochemically. A total of 35 (30.9%) samples were found to be positive by RT-PCR, with 15 (42.8%), 9 (25.7%), 5 (14.2%), 4 (11.4%), 1 (2.8%), and 1 (2.8%) of them being identified as C. abortus, B. melitensis, S. Abortusovis, C. burnetii, L. monocytogenes and Campylobacter spp., respectively. The presence of the antigen was confirmed also immunohistochemically in the cases with B. melitensis and L. monocytogenes. As a consequence, C. abortus was found to cause the highest rate of sheep abortion cases, which should be taken into account when implementing control measures in epidemiological investigations.