Effect of administration of sodium fluoride in different concentrations on Gpx1 gene expression in NRK-52E cell


Usta A., Yüksek V., Çetin S., Dede S.

10th International Medical and Health Sciences Research Congress (10.UTSAK-2022), Ankara, Turkey, 27 - 28 August 2022, vol.1, no.1, pp.621-622

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.621-622
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Glutathione peroxidase is one of the most important antioxidant enzymes that functions in the detoxification of hydrogen peroxide. Glutathione peroxidase 1 is an enzyme encoded by the Gpx1 gene. There are some risks of taking large amounts of fluoride into the body. Since the kidneys are the primary excretion site of orally ingested fluoride, the kidneys are exposed to more fluorine than other organs. This study was carried out to investigate the effects of fluorine at different concentrations on the Gpx1 gene in NRK-52E kidney cell line and thus to elucidate its effects on antioxidant status. NaF concentrations to be applied to NRK-52E cells were determined by MTT test. The proliferation concentration of NaF was determined as 10 μM, IC25 dose 2250 μM, IC50 dose 4250 μM in cells exposed to sodium fluoride for 24 hours. Four groups were formed as control, proliferative NaF, IC25 NaF, IC50 NaF groups. Substances were applied to cells and total mRNA was isolated. Expression levels of target gene were determined by RT-qPCR method. Compared to the control group, a decrease in Gpx1 gene expression was observed as the fluoride concentration increased. A decrease in Gpx1 gene expression was observed with increasing fluoride concentration compared to the control group. It was thought that decreased expression of the antioxidant gene Gpx1 may be among the causes of cell damage caused by fluorine in the NRK-52E cell line. It was concluded that glutathione peroxidase activity may be inhibited because fluorine increases hydrogen peroxide radicals in NaF IC25 and IC50 groups.