Characterization of bovine and human group B streptococci isolated in Turkey


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Ekin I. , Gurturk K.

JOURNAL OF MEDICAL MICROBIOLOGY, cilt.55, ss.517-521, 2006 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 55 Konu: 5
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1099/jmm.0.46156-0
  • Dergi Adı: JOURNAL OF MEDICAL MICROBIOLOGY
  • Sayfa Sayıları: ss.517-521

Özet

In the study, group B streptococci (GBS) isolated from bovines and humans in and around Van, eastern Turkey, were serotyped, and their haemagglutination and lectin-agglutination properties were also determined. This study is the first epidemiological survey of GBS serotypes performed in Turkey. A total of 148 GBS isolates, 76 from bovine milk and 72 from women attending a maternity polyclinic, were examined by co-agglutination, slide haemagglutination and slide lectin-agglutination tests. By the co-agglutination test, 34 (44.7%) of bovine isolates and 49 (68%) of human isolates could be serotyped. In bovine isolates, type VII (11.8%), III (10.5%) Ic (6.5%) and VIII (3.9%) were the most frequently detected serotypes. The most frequent human serotypes were Ic (33.3%), IV (8.3%), VIII (6.9%), V (5.5%) and R (5.5%). In the haemagglutination test using rabbit erythrocytes, 23 (33.3%) bovine and 15 (23.4%) human isolates were found to be positive. The bovine GBS isolates showed a significant positive agglutination reaction with Dolichos biflorus lectin (30.4%), whereas the human GBS isolates were found to be positive for Arachis hypogea (18.8%) and Canavalia ensiformis (37.5%) lectins. The treatment of GBS with trypsin was also found to be important for the demonstration of the haemagglutination and lectin-agglutination properties of GBS. The results of the study provide data on serotype distribution and the formulation of a possible GBS vaccine in Turkey, and the lectin-agglutination tests may also be useful for differentiating bovine and human GBS strains.
  • In the study, group B streptococci (GBS) isolated from bovines and humans in and around Van, eastern Turkey, were serotyped, and their haemagglutination and lectin-agglutination properties were also determined. This study is the first epidemiological survey of GBS serotypes performed in Turkey. A total of 148 GBS isolates, 76 from bovine milk and 72 from women attending a maternity polyclinic, were examined by co-agglutination, slide haemagglutination and slide lectin-agglutination tests. By the co-agglutination test, 34 (44·7?%) of bovine isolates and 49 (68?%) of human isolates could be serotyped. In bovine isolates, type VII (11·8?%), III (10·5?%), Ic (6·5?%) and VIII (3·9?%) were the most frequently detected serotypes. The most frequent human serotypes were Ic (33·3?%), IV (8·3?%), VIII (6·9?%), V (5·5?%) and R (5·5?%). In the haemagglutination test using rabbit erythrocytes, 23 (33·3?%) bovine and 15 (23·4?%) human isolates were found to be positive. The bovine GBS isolates showed a significant positive agglutination reaction with Dolichos biflorus lectin (30·4?%), whereas the human GBS isolates were found to be positive for Arachis hypogea (18·8?%) and Canavalia ensiformis (37·5?%) lectins. The treatment of GBS with trypsin was also found to be important for the demonstration of the haemagglutination and lectin-agglutination properties of GBS. The results of the study provide data on serotype distribution and the formulation of a possible GBS vaccine in Turkey, and the lectin-agglutination tests may also be useful for differentiating bovine and human GBS strains.