Enzymatic properties of group B streptococci (GBS) serotypes from bovine milk and human routine vaginal specimens were investigated. Out of the 56 human and 66 bovine GBS, 35 and 30 could be classified serologically by a co-agglutination test with type-specific antisera, respectively. Hyaluronidase (HYAL), streptokinase (SK) and protease activities were detected using culture media. HYAL activity was observed mostly in typable human GBS, and serotypes la, lc and II comprised 77.3 % of the typable strains producing HYAL. Bovine GBS serotypes II, III and VII comprised 87.5 % of typable bovine strains exhibiting HYAL activity. SK activity was detected only in three human GBS. Human GBS serotypes la, lc, II, III, VII and almost all typable bovine GBS strains showed protease activity. beta-D-glucosidase activity was frequently observed in human GBS, whereas N-acetyl-beta-D-glucosaminidase activity was mostly detected in non-typable GBS from humans. These results indicate that different GBS serotypes could vary in their virulence properties, and bovine and human GBS isolates could not be differentiated by their enzyme activities. Use of the culture media appeared to be a simple-to-apply and useful method for the detection of extracellular enzyme activity such as HYAL, protease and SK.