Dual Responsive Disposable Electrode for the Enumeration ofEscherichia coliin Whole Blood


Panhwar S., İLHAN H., Hassan S. S. , Zengin A. , BOYACI İ. H. , TAMER U.

ELECTROANALYSIS, vol.32, no.10, pp.2244-2252, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 10
  • Publication Date: 2020
  • Doi Number: 10.1002/elan.202060185
  • Title of Journal : ELECTROANALYSIS
  • Page Numbers: pp.2244-2252
  • Keywords: Disposable electrode, spectro-electrochemical labels, Cyclic Voltammetry, Pathogens bacteria, blood, GOLD NANOPARTICLES, ELECTROCHEMICAL DETECTION, ESCHERICHIA-COLI, E. COLI, BIOSENSOR, BACTERIA, O157H7, SENSOR

Abstract

In this report, we have designed a dual responsive disposable electrode for the enumeration ofEscherichia coli K12 (E. coli K12). The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal-organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro-electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantifyE. coliwith a ranging from 10(1)to 10(7) cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.