This study was carried out to determine the effects of humic acid applications to some nutrient concentrations of soybean cultivars (Stressland, Cisne and 54240) under the greenhouse conditions in 2010 year. In study, the effect of different humic acid (control, 400,800 and 1200 ml/100 kg seed) applications and soybean cultivars (Cisne, Stressland and 54240) on mineral ions content in various plant organs of soybean (Glycine max. L.) was investigated. Soybean plant was divided into leaf, shoot and root parts for nutrient (Ca, Na, K, P, Fe, Mn, Cu and Zn) concentration measurements and were analysed. Results indicated that the nutrient concentration in plant tissues like leaf, shoot and root of soybean was strongly affected by all humic acid treatments. Usually, nutrient concentration in all parts of plants was increased with in creasing of humic acid doses. It was determined that increased Ca, Na, K, P and Fe contents while decreased Mn, Cu and Zn contents in plant organs of soybean after 800 ml/100 kg humic acid application.