Detection and Sequence Analysis of Equine Gammaherpesviruses from Horses with Respiratory Tract Disease in Turkey


Ataseven V. S. , Bilge-Dagalp S., Oguzoglu T. C. , Karapinar Z. , Guzel M., Tan M. T.

TRANSBOUNDARY AND EMERGING DISEASES, cilt.57, ss.271-276, 2010 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 57 Konu: 4
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1111/j.1865-1682.2010.01146.x
  • Dergi Adı: TRANSBOUNDARY AND EMERGING DISEASES
  • Sayfa Sayısı: ss.271-276

Özet

P>The equid herpesvirus 2 (EHV-2) and 5 (EHV-5), identified agents of respiratory infections and keratoconjunctivitis cases in some equids, comprise a high degree of antigenic heterogeneity. Prevalence and genetic characterization of EHV-2 and EHV-5 strains from Turkey were investigated in this study. A total of 73 nasal swabs and 54 blood specimens were sampled from horses with respiratory tract diseases characterized by mucopurulent nasal discharge and occasional coughing. Overall, EHV-2- and EHV-5-specific DNA amplicons were obtained from 19.2% (14/73) and 21.9% (16/73) of horses tested by multiplex nested PCR. Sequences of EHV-2 and EHV-5 glycoprotein B (gB) gene were used in a phylogenetic analysis that included six EHV-2 and three EHV-5 isolates, which showed that the Turkish EHV-2 and EHV-5 strains have marked sequence divergence from European strains and from each other. Turkish EHV-2 isolates were divided into two distinct subdivisions, and a few isolates were located on a separate branch. This study provides the first epidemiological and phylogenetical report about EHV-2 and EHV-5 infections in Turkey.