Cherry leafroll virus in Juglans Regia in the Lake Van basin of Turkey

Creative Commons License

Ozturk M., Sıpahıoglu H., Ocak M., Usta M.

Journal of Plant Pathology, cilt.90, sa.1, ss.75-79, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 90 Sayı: 1
  • Basım Tarihi: 2008
  • Dergi Adı: Journal of Plant Pathology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.75-79
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Walnut orchards of the Lake Van basin (Turkey)
were surveyed from June to October 2006 to determine
the incidence of viral infections. ELISA and RT-PCR
were used to investigate the presence of Cherry leaf roll
virus (CLRV) and Plum pox virus (PPV), testing a total
of 870 samples collected from traditional seed-grown
plantations. Whereas no PPV was detected in any of the
samples, CLRV was found for the first time in the surveyed locations with an average incidence of 12.9%.
Two viral isolates from Edremit were mechanically
transmitted to Chenopodium amaranticolor in which
they caused local chlorotic spots followed by development of small leaves and apical necrosis. A 366 bp DNA
fragment was amplified by RT-PCR from the 3’ noncoding region of RNA-2 of both viral isolates and sequenced. Isolate Edremit-2 was 93-98% identical to the
comparable sequences of other isolates for which information is available, whereas isolate Edremit-1 had a
lower sequence identity (53-46%). The size of the coat
protein subunits of both viral isolates was 52.4 kDa as
determined by electrophoresis.