Research Information System
INSAC Natural and Health Sciences (INHS-2020), İstanbul, Turkey, 22 - 23 May 2020, vol.1, no.1, pp.439
INSAC Natural and Health Sciences (INHS-2020)
440 Proceedings
Book pp: 439
Growth
Hormone (GH) Gene Polymorphism in Norduz Sheep
Nzar
Ibrahim Yousuf1, Hasan Koyun1
1Department of Animal Sciences of Agricultural Faculty, Van
-Yuzuncu Yil University, Van, Turkey.
ibrahimnzar22@gmail.com,
hkoyun@yyu.edu.tr
Abstract:
Norduz sheep is
a local sheep breed, raised for meat, milk, offspring and fleece traits in
rural areas in the region of Norduz in Gürpınar district of Van province of
Turkey. The PCR-based method is one of the techniques, associating
amplification of a conserved DNA region, yielded PCR products with restriction
endonucleases throughout the livestock genomes. Based on the resulting
polymorphisms one can detect desired genotypes using statistical methods among
yielded alleles for Marker -assisted selections (MAS). The aim of the study was
to identify growth hormone (GH/MspI) gene polymorphisms and to determine its
associations with six growth traits in Norduz sheep. Three different loci
(GHY1, GHY2, and GHY3) of GH gene on chromosome 11 of sheep genome were
selected to seek out polymorphisms among individuals by using PCR-RFLP
technique. After digesting, the three loci of GH gene (599, 690, and 679 bp)
with MspI enzyme no polymorphisms were detected in this study. The 50
individuals of Norduz sheep displayed only AB genotypes for all three loci,
therefore indicating that there were no polymorphisms present within the
investigated regions of GH gene.
Acknowledgements:
This research
was financially supported by the Scientific Research Center (BAP) of
Van-Yuzuncu Yil University with the project number: FYL-2020-8221.
Key
words: Norduz
sheep, Growth hormone (GH)gene, PCR-RFLP, Marker-assisted selection (MAS)
INSAC Natural and Health Sciences (INHS-2020)
440 Proceedings
Book pp: 439
Growth
Hormone (GH) Gene Polymorphism in Norduz Sheep
Nzar
Ibrahim Yousuf1, Hasan Koyun1
1Department of Animal Sciences of Agricultural Faculty, Van
-Yuzuncu Yil University, Van, Turkey.
ibrahimnzar22@gmail.com,
hkoyun@yyu.edu.tr
Abstract:
Norduz sheep is
a local sheep breed, raised for meat, milk, offspring and fleece traits in
rural areas in the region of Norduz in Gürpınar district of Van province of
Turkey. The PCR-based method is one of the techniques, associating
amplification of a conserved DNA region, yielded PCR products with restriction
endonucleases throughout the livestock genomes. Based on the resulting
polymorphisms one can detect desired genotypes using statistical methods among
yielded alleles for Marker -assisted selections (MAS). The aim of the study was
to identify growth hormone (GH/MspI) gene polymorphisms and to determine its
associations with six growth traits in Norduz sheep. Three different loci
(GHY1, GHY2, and GHY3) of GH gene on chromosome 11 of sheep genome were
selected to seek out polymorphisms among individuals by using PCR-RFLP
technique. After digesting, the three loci of GH gene (599, 690, and 679 bp)
with MspI enzyme no polymorphisms were detected in this study. The 50
individuals of Norduz sheep displayed only AB genotypes for all three loci,
therefore indicating that there were no polymorphisms present within the
investigated regions of GH gene.
Acknowledgements:
This research
was financially supported by the Scientific Research Center (BAP) of
Van-Yuzuncu Yil University with the project number: FYL-2020-8221.
Key
words: Norduz
sheep, Growth hormone (GH)gene, PCR-RFLP, Marker-assisted selection (MAS)