INSAC Natural and Health Sciences (INHS-2020)

440 Proceedings Book  pp: 439

Growth Hormone (GH) Gene Polymorphism in Norduz Sheep

Nzar Ibrahim Yousuf1, Hasan Koyun1

1Department of Animal Sciences of Agricultural Faculty, Van -Yuzuncu Yil University, Van, Turkey.

ibrahimnzar22@gmail.com, hkoyun@yyu.edu.tr

Abstract: Norduz sheep is a local sheep breed, raised for meat, milk, offspring and fleece traits in rural areas in the region of Norduz in Gürpınar district of Van province of Turkey. The PCR-based method is one of the techniques, associating amplification of a conserved DNA region, yielded PCR products with restriction endonucleases throughout the livestock genomes. Based on the resulting polymorphisms one can detect desired genotypes using statistical methods among yielded alleles for Marker -assisted selections (MAS). The aim of the study was to identify growth hormone (GH/MspI) gene polymorphisms and to determine its associations with six growth traits in Norduz sheep. Three different loci (GHY1, GHY2, and GHY3) of GH gene on chromosome 11 of sheep genome were selected to seek out polymorphisms among individuals by using PCR-RFLP technique. After digesting, the three loci of GH gene (599, 690, and 679 bp) with MspI enzyme no polymorphisms were detected in this study. The 50 individuals of Norduz sheep displayed only AB genotypes for all three loci, therefore indicating that there were no polymorphisms present within the investigated regions of GH gene.

Acknowledgements: This research was financially supported by the Scientific Research Center (BAP) of Van-Yuzuncu Yil University with the project number: FYL-2020-8221.

Key words: Norduz sheep, Growth hormone (GH)gene, PCR-RFLP, Marker-assisted selection (MAS)