Determination of organophosphate resistance status and mechanism in Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) from Turkey

Karaagac S. U., Konuş M.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.40, no.5, pp.417-422, 2015 (SCI-Expanded) identifier identifier


Objective: The objectives of this study were to determine resistance status to malathion and pirimiphos-methyl insecticides and to make biochemical analysis of resistance mechanism(s) developed to these insecticides in Sitophilus zeamais (S. zeamais) populations, collected from two different locations in Turkey. Two organophosphate insecticides, malathion and pirimiphos-methyl, were examined by bioassay using a discriminating dosage technique with impregnated filter papers. Mortality percentages were determined at the discriminating doses of these insecticides. In addition, esterase, glutathione S-transferase, and cytochrome P450 monooxygenase activities were also determined in this study in order to analyze detoxification mechanism(s) of tested insecticides in S. zeamais.