Effect of antioxidant vitamins on inflammation in rat liver tissue with fluorosis

Usta A., Öner A. C.

6th International Congress on Veterinary and Animal Sciences (6.ICVAS-2021), Hatay, Turkey, 2 - 04 September 2021, pp.117-118

  • Publication Type: Conference Paper / Summary Text
  • City: Hatay
  • Country: Turkey
  • Page Numbers: pp.117-118
  • Van Yüzüncü Yıl University Affiliated: Yes


Objective: Excess Fluorine induces oxidative stress and production of inflammatory cytokines. In this study, it was aimed to investigate the possible effects of vitamins C and E on inflammation gene expressions in liver tissues, which are thought to be the most affected in rats with fluorosis.

Materials and Methods: 48 Wistar-Albino male rats weighing 200–250 g were used in this study. 6 groups including groups Control (K), corn oil (M), NaF (F), NaF together with vitamin E (F+E), vitamin C (F+C), vitamin C+E (F+C+E) has been created. 150 mg/kg/day was added to the drinking water of the NaF groups for 16 weeks. Since Vitamin E is given by dissolving in corn oil, the corn oil group was also created. These rats were given 0.2 ml/day of corn oil. After giving NaF for 16 weeks to the treatment groups, vitamin C (100 mg/kg/day), vitamin E (300 mg/kg/day), vitamin C+vitamin E (100 mg/kg/day+300 mg/kg/day) for 4 weeks ) was administered orally. At the end of the experiment, all groups were sacrificed and liver tissues were taken. Expression levels of TNF-α and IL-1β genes, which are inflammation markers, were determined by real time-qPCR in RNA isolation products obtained from rat liver tissue. Beta-actin (Actb) was used as the internal control gene.

Results: TNF-α expression levels were highest in the NaF group. It was significantly lower in all other groups. IL-1β gene expression levels were found to be at the control level in the corn oil group, lower than the control in the vitamin treatment groups, and highest in the NaF group.

Conclusion: It was observed that the application of antioxidant vitamins for the treatment of possible liver damage caused by fluoride reduced inflammation. It was determined that vitamin C, vitamin E, vitamin C+E had significant beneficial effects on inflammation markers. It was observed that the combination of vitamin C+E was more effective in reducing inflammation compared to the groups given therapeutic vitamin C and vitamin E for proinflammatory cytokines such as IL-1β and TNF-α, which increase in fluorosis.

Key words: Antioxidant vitamin, fluorosis, gene expression, inflammation