Plant-mediated synthesis of silver nanoparticles (Ag NPs) has increased its use in various biomedical applications due to its cost-effectiveness, renewable and environmentally friendly properties. Therefore, the focus of the study was to synthesize Ag NPs and to evaluate their antibacterial, and antioxidant activities by using the aqueous root extract of Ferula pseudodalliacea plant as a reducing agent. In addition, the synthesis of nanoparticles was confirmed by performing Ag NPs/Fp characterization processes. According to the findings of our research, color change due to surface plasmon resonance was confirmed by UV-vis spectrometry. The crystal properties of Ag NPs/Fp were determined according to the XRD model. Phytochemicals responsible for coating and reduction of Ag NPs/Fp were observed by FT-IR analysis. It has been shown that the therapeutic effect of Ag NPs/Fp exhibits better antimicrobial and antioxidant activity than aqueous extract. In addition, it was determined that Ag NPs/Fp structures showed the best antifungal effect against Candida albicans ATTC 90028 pathogen and gave a better zone than Rifampicin antibiotic. Therefore, studies at the molecular level and more comprehensively are required to determine the bioactive components and pharmacological effects of Ferula pseudodalliacea plant to confirm our antibacterial, antioxidant, and antifungal activity results.