Diplotaenia turcica is a plant species which is found in Bitlis-Van-Hakkari region and used in traditional treatments due to its medical and protective activities. Aim of this study is to investigate effects of root extract of Diplotaenia turcica plant on rat over tissue with histological and stereological methods. Four groups, with seven rats each, were used in experiments. Experimental groups were as follows; (I): control group, standard food and water was administered, Diplotaenia turcica plant root extract groups; (II): 250 mg/kg, (III): 550 mg/kg, (IV): 1000 mg/kg were administered as single dose/day with gastric gauge for 28 days. At the end of the experiments rats were decapitated and right over tissue was obtained. Histologically an increase in over medulla structure and an increase in corpus luteum and Graaf follicle number in cortex in plant administered groups were observed. Total volume comparison showed no difference between groups (p>0.05). When follicle numbers were evaluated Diplotaenia turcica administered groups showed a significant decrease in Primordial follicle number compared to control (p<0.05). No change in Primary follicle number (p>0.05) and an increase in Graaf follicle number (p<0.05) was observed. These results showed that Diplotaenia turcica increases follicular maturation by acting on this process.