Inhibition effect of nicotinamide (vitamin B-3) and reduced glutathione (GSH) peptide on angiotensin-converting enzyme activity purified from sheep kidney

Baş Z.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.189, ss.65-71, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 189
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.ijbiomac.2021.08.109
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.65-71
  • Anahtar Kelimeler: Angiotensin-converting enzyme (ACE), Nicotinamide (vitamin B-3), Reduced glutathione (GSH), Antioxidant, Purification, nhibition, OXIDATIVE STRESS, BLOOD-PRESSURE, HYPERTENSION, SUPPLEMENTATION, PURIFICATION, CAPTOPRIL, ANTIOXIDANT, AFFINITY, THERAPY, ASSAY
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Angiotensin-converting enzyme (ACE, EC 3.4.15.1) plays a significant role in blood pressure regulation and inhibition of this enzyme is one of the significant drug targets for the treatment of hypertension. In this work, ACE was purified from sheep kidneys with the affinity chromatography method in one step. The purity and molecular weight of ACE were designated using the SDS-PAGE method and observed two bands at around 60 kDa and 70 kDa on the gel. The effects of nicotinamide (vitamin B-3) and reduced glutathione (GSH) peptide on purified ACE were researched. Nicotinamide and GSH peptide on purified ACE showed an inhibition effect. IC50 values for nicotinamide and GSH were calculated as 14.3 mu M and 7.3 mu M, respectively. Type of inhibition and K-i values for nicotinamide and GSH from the Lineweaver-Burk graph were determined. The type of inhibition for nicotinamide and GSH was determined as non-competitive inhibition. Ki value was calculated as 15.4 mu M for nicotinamide and 6.7 mu M for GSH. Also, GSH peptide showed higher inhibitory activity on ACE activity than nicotinamide. In this study, it was concluded that nicotinamide and GSH peptide compounds, which show an inhibition effect on ACE activity, may have both protective and therapeutic effects against hypertension.