8. Ulusal Veteriner Biyokimya ve Klinik Biyokimya Kongresi (8.Vetbiyokimya-2016), Bursa, Turkey, 22 - 24 September 2016, vol.1, no.1, pp.164-165
Objective: This study was planned in order to effect of thymoquinone (TQ) known pro inflammatory and antioxidant proporties the potential oxidative DNA damage and reveals the effects of antioxidant system in high glucose added kidney cell line.
Material and Methods: BHK-21 cellswere cultured with the regular passages. MTT cell viability was determined of IC50 for TQ and glucose. Cells were seed into plates.Control, glucose (285mM), TQ (10uM), and its cross groups were prepared. Cells were used for analysis after hours 24 incubation. In these examples; 8-OHdG, TAS, TOS were analyzed by ELISA.
Results: The 8-OHdG levels in TQ+glucose (p≤0.05) group were higher than control and other groups. TQ group’s levels (p≤0.05) decreased according to glucose group. TAS levels of TQ group (p≤0.05) were higher than other groups, but, this was not statistically significant. TOS (p≤0.05) increased in all groups compared to the control. However, no significant difference was found between study groups. While OSI value increased in all groups (p≤0.05) compared to controls, only in the group given TQ group (p≤0.05) were lower than glucose and glucose+TQ.
Conclusions: Consequently, the TQ treatment on the high glucose added BHK-21 cell line were obtained that high oxidative DNA damage and TOS and unchanged TAS levels. The high glucose added groups had higher OSI index than TQ and control groups.
Keywords: glucose, cell culture, thymoquinone, DNA damage, TAS/TOS