In sustainable agriculture, seeking eco-friendly methods to promote plant growth and improve crop productivity is a priority. Humic acid (HA) and plant growth promoting rhizobacteria (PGPR) are among the most effective methods that utilize natural biologically-active substances. The aim of the present study was to analyze the effect of the presence of HA on potato (Solanum tuberosum L.) inoculation with PGPR (Bacillus megatorium and Bacillus subtilis) when compared to control and recommended doses of NPK. Seed tubers treated by humic acid (200, 400, and 600 kg ha(-1)) and PGPR, separately or in combination, and NPK (50% and 100%) were planted into soil and untreated soil. Treatments were assessed for plant growth, classified tuber yields, quality, and mineral contents of potato tubers. There were highly significant increases in potato growth, tuber yields, and quality in PGPR and HA inoculated crops. Tuber size, weight, specific gravity, dry matter, starch, protein, and mineral contents (except Cu) were improved with PGPR treatments and further increased when administered with humic acids. Inoculation with PGPR mixed culture and 400 kg ha(-1) HA increased total potato tuber yield by about 140% while conventional single treatment of 100% NPK fertilizer only led to an increase in potato production of 111% when compared to the control. The results demonstrated that this integrated approach has the potential to accelerate the transformation from conventional to sustainable potato production.