Preparation of molecularly imprinted PDMS elastomer for selective detection of folic acid in orange juice


Zengin A., Badak M. U. , Bilici M., SULUDERE Z., Aktaş N.

APPLIED SURFACE SCIENCE, vol.471, pp.168-175, 2019 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 471
  • Publication Date: 2019
  • Doi Number: 10.1016/j.apsusc.2018.12.008
  • Journal Name: APPLIED SURFACE SCIENCE
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.168-175
  • Keywords: Molecularly imprinted polymers, Poly(dimethylsiloxane) elastomer, Folic acid, Surface initiated RAFT polymerization, SOLID-PHASE EXTRACTION, PASTE ELECTRODE, VOLTAMMETRIC DETERMINATION, SURFACE MODIFICATION, POLYMERS, NANOPARTICLES, RECOGNITION, PERFORMANCE, ADSORPTION, POLYMERIZATION

Abstract

Herein, it is reported an effective method to prepare novel molecularly imprinted polymers (MIP) on poly(dimethylsiloxane) (PDMS) elastomer via a combination of non-covalent imprinting approach and surface initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization for sensitive and selective detection of folic acid (FA). For this purpose, 2-(2-Methoxyethoxy) ethyl methacrylate (MEOMA), ethylene glycol dimethacrylate (EGDMA), azobisisobutyronitrile (AIBN), FA and dimethylsulfoxide (DMSO) were used as functional monomer, cross-linker, initiator, template molecule, and porogen, respectively. The adsorption behavior followed the Scatchard equation between FA and PDMS-MIP with a saturation adsorption capacity of 4.51 mg/g and pseudo-second-order kinetics with 60 min equilibrium adsorption time. Furthermore, PDMS-MIP elastomer was successfully applied for selective extraction and detection of FA from orange juice with sufficient recovery (95.5-100.5%) and relative standard deviation less than 7.0%. The limit of detection (LOD) for FA was found to be 0.0031 mu g/mL with a linear range between 0.01 and 100 mu g/mL and a correlation coefficient of 0.9997. Results showed the proposed method could easily, efficiently and selectively extracted FA from complex media. Note that this novel proposed method will open a new way to detect any selected molecules such as pesticide, protein, drug, etc. using molecularly imprinted PDMS elastomer materials.