Akademik Veri Yönetim Sistemi
International Congress of Medical and Health Sciences Studies, Ankara, Türkiye, 13 - 14 Aralık 2022, ss.96
Bisphenol
A (BPA) is a chemical component used in plastic products around the world. This
study aims to examine the effects of these chemical compounds to which humans
are frequently exposed in everyday life, on the heterogeneity and distribution
of mast cells in the gastrointestinal system. For the study, 24 male Wistar
albino rats were divided into 4 groups (control, sham, 25 mg/kg, and 50 mg/kg
BPA treated). BPA was dissolved in corn oil and administration was done by oral
gavage for thirty days. Gastrointestinal tissue samples taken from animals
anesthetized with inhalation anesthesia were fixed using BLA (Basic Lead
Acetate) and Carnoy fixation. Then, following routine tissue follow-up, they
were blocked with paraplast. Sections (6 μm) taken from the blocks were stained
using Toluidine blue (TB) and Alcian blue-safranine O 8GX (AB-SO) combined
dyes. Counting and statistical analysis of the mast cells in the TB-stained
sections were performed. According to the results of the analysis, a higher
number of mast cells was observed in the BLA fixation solution, while the
increase in the number of mast cells was statistically significant in the
groups treated with BPA (P<0.05). In the combined AB-SO 8GX staining
results, AB (+), S (+), and Mix (+) mastocytes were found in almost all
sections of the digestive tract. As a result of this staining process, again,
no difference was found in the semi-quantitative evaluations performed
including the groups treated with BPA. Although BPA does not affect the
heterogeneity of mast cells, it does affect their distribution. Therefore, it
is assumed that further studies will need to be carried out.